Reticulin stain in histopathology pdf

Feb 22, 2011 special stains, such as reticulin stain and cd34 immunostain, are very helpful in the diagnosis of well differentiated hepatocellular carcinoma hcc. A reticulin stain, however, may reveal doublelayered cell plates and layers of reticulin fibers at the edge of the specimen fragments, both of which are features of cirrhotic nodules. The reticulin stain is performed on bone biopsy sections for detecting the presence, assessing the severity and identifying specific disease. The value of this stain in detecting a hitherto unsuspected fungal infection is highlighted in the case described. An intro to routine and special staining in histopathology. Gomoris silver impregnation staining technique for reticulin. Substance stain component stains elastic fibers verhoeff van gieson collagen red muscle, elastin, reticulin yellow nuclei blue black elastic fibers in large vessels aorta reticulin fibers reticulin stain reticulin fibers black demonstrates reticular fibers and basement membrane material mallory ptah martius scarlet blue van gieson. Diagnostic value of immunohistochemical staining of gp73. Liver anatomy and histology comparative pathology laboratory division of comparative medicine arlin rogers.

Special stains in histopathology linkedin slideshare. Conns biological stains, 9th edition williams and wilkins please refer to the msds for chemical and safety information reticulin stain kit in the u. A liver biopsy stained using the reticulin demonstrating the normal hepatic plate thickness and mild steatosis. Introduction to laboratory stains dr chandika epitakaduwa mb bscolombo,d path,mdhistopathology acting. Feb 03, 2015 special stain in histopathology presented by dr. Due to the excellent differentiation of collagen, elastic, fibrin and muscle fibers, the movat can reveal subtle changes that the routine and normal special stains do not. All surrounding nontumor liver cells and 21 of 29 72. Often not helpful in nonclassic cases outlines architecture of liver and spleen to diagnose hemangiopericytoma, vascular smooth muscle, fibrosarcoma or fibrothecoma stains each cell vs. It is used to study heart tissue, blood vessels and vascular and lung diseases. A reticulin stain, however, may reveal doublelayered cell plates and layers of reticulin fibers at the edge of the specimen fragments, both. By colouring otherwise transparent tissue sections, these stains allow highly trained pathologists and researchers to view, under a microscope, tissue morphology structure or to look for the presence or prevalence of particular cell types, structures or even microorganisms such as. Role of special stains in diagnostic liver pathology.

This is a helpful stain to use when the diagnosis of hepatocellular carcinoma is being entertained sometimes we are fortunate to see the classic features of neoplasia like hyperchromacia, increased nuclear to cytoplasmic ratio, or cellular. In pathology, the reticulin stain, is a popular staining method in histology. The manuals for this section have been compiled by susan l. In routine circumstances, however, the stain was of. Figure 1 reticulin stain showing normal liver parenchyma with a portal tract in the top left of the image p and a central vein in the bottom right v. This stain uses carbolfuchsin to stain the lipid walls of acid fast organisms such as m. The first step in the staining procedure consists of oxidation of the hexose. Diana harrington, bs, htascp and carla shoffeitt, msm, htascp this course is the second in a series of courses that will explore special stains used in the clinical histology laboratory. Eighty percent of these tumors recur, and 23% metastasize. These manuals are in adobe acrobat pdf format and require the acrobat reader for viewing. The most commonly used method is the ziehlneelsen method, though there is also a kinyouns method. It is the preliminary or the first stain applied to the tissue sections gives diagnostic information in most cases. In the liver, such fibers are present as part of the extracellular matrix in the space of disse. Mckeever, in diagnostic immunohistochemistry third edition, 2010.

This histology stain uses light green, iron hematoxylin, and acid fuchsin. The hepatocyte cords are focally expanded h, and a bandlike area of reticulin collapse arrows near the central vein cv highlights the necrosis magnification 200. Robbins and cotran pathologic basis of disease 2010, 8th ed. Evaluation of bone marrow reticulin formation in chronic.

Reticulin staining employs the use of silver impregnation of a section to highlight reticulin fibers type iii collagen. The reticulin stain is useful in parenchymal organs such as liver and spleen to outline. Movat pentachrome is a super connective tissue stain. With the use of stains and dyes, histology allows researchers to visualize particular tissue structures, chemical elements within cells, tissues and even microorganisms.

This chapter discusses all these connective tissue fibres along with the different stains to elucidate them. Reticulin stain kit is a metal impregnation technique where. Role of special stains in diagnostic liver pathology murli krishna, m. Gordon and sweets technique demonstrates reticulin fibres using an argyrophiltype reaction involving silver solutions. High concordance in grading reticulin fibrosis and.

Due to the excellent differentiation of collagen, elastic, fibrin and muscle fibers, the movat can reveal subtle. The reticulin stain demonstrates zellballen phenomenon in the paraganglioma with appropriate immunoperoxidase staining characteristics table 124. May 11, 2020 reticulin staining employs the use of silver impregnation of a section to highlight reticulin fibers type iii collagen. Carcinomas have reticulin around clusters of cells. The reticulin stain in bone marrow biopsies beyond marrow. Hemangiopericytoma an overview sciencedirect topics.

Pdf files contains the procedure for a special stain, a procedure card, and sample container labels for the. This 10 component comprehensive reticulin stain kit is excellent stain for the identification. A reticulin stain reveals the marked deposition of collagen within the lumen of the central vein. Reticulin is a normal component of the bone marrow stroma and can be detected with a reticulin stain in 73% to 81% of healthy subjects. Special stains, such as reticulin stain and cd34 immunostain, are very helpful in the diagnosis of well differentiated hepatocellular carcinoma hcc. A complete course on the concepts and skills needed for a biomedical scientist to work within a functioning histopathology laboratory. Basement membrane is the connective tissue elements that separate the epithelial and endothelial cells from the underlying connective tissue. In routine circumstances, however, the stain was of little diagnostic use, as the. The advent and evolution of histology follows that of microscopy as outlined in a very short history of histology. The reticulin stain is useful in parenchymal organs such as liver and spleen to outline the architecture. They stain with pas but not with alcian blue, colloidal iron, mucicarmine. A complete course on the concepts and skills needed for a biomedical scientist to. Unusual reticulin staining pattern in welldifferentiated. Kem hospital, 2012 sheehan and hrapchak, theory and practice of histotechnology connective tissue 2nd edition 10.

Pdf exploratory study of special stains for enhancing histological. Special stains gordon sweet reticulin histological techniques. Reticulin stain highlighting the reticulin pattern in acute hepatic necrosis. These techniques rely on the impregnation of the fibres by silver ions and subsequent reduction of these silver ions to their visible metallic form. In addition, changes in reticular pattern can be seen in some liver diseases. Histopathology special stain reticulin test for surgical. It is used to visualize reticular fiber and used extensively in liver histo pathology. There are rare occurrences of metastases of known neoplasms from other sites to the middle ear and temporal bone that histologically simulate adenomacarcinoid see later discussion. In the liver, both necrosis and cirrhosis cause irregular patterns of reticulin. Research in histochemistry, palo alto reticulin stain why collapse of reticulin fibers. Chapter 2 general oversight stains for histology and histopathology 29.

But histopathology relies heavily on optical technology, and microscopy is covered in detail from fixation, through staining to light and electron imaging methods. Figure 2op lefthaematoxylin van gieson hvg stain showing mild zone 3 steatosis without fibrosis, in which collagen fibres pinkred, t. Crookham, j, dapson,r, hazardous chemicals in the histopathology laboratory. These individuals provide the basis for successful scientific collaborations, rapid concept advancements, unparalleled consultation services, and expert dissemination of information and findings to regulatory and scientific bodies. Hemangiopericytoma who grade ii or iii hemangiopericytoma is a highly cellular and mitotically active neoplasm that is rich in pericellular reticulin and stainable with antitype iv collagen. Before ihc, reticulin was used to differentiate sarcomas from carcinomas. Special stain in histopathology linkedin slideshare.

Reticulin stains are silver stains based on the argyrophilic properties of reticulin fibers. Special stains, immunohistochemistry and genomic testing. Review special stains in diagnostic liver pathology krishna. Reticulin patterns were examined in 25 consecutive malignant melanomas, 25 benign cellular naevi and 10 spitz benign juvenile naevi. Recent advances in the classification of hepatocellular. Jan 06, 2020 gomoris silver impregnation staining technique for reticulin fibers by editorial team on january 6, 2020 in histopathology reticular fibre is the type of connective tissue composed of type iii collagen secreted by reticular cells. The term special stains has long been used to refer to a large number of alternative staining techniques that. Absolute features were that a completely intact basement membrane was a strong indicator of benignity and that pericellular reticulin was evidence of subsequent benign behaviour. Reticulin staingomoris method reticulin stain demonstrate both reticular fibers and basement membrane material. Reticulin stain uses silver impregnation to detect reticulin fibers, which are made of type 3 collagen. Dr lal pathlabs offers test service for histopathology special stain reticulin test for checking surgical pathology. Reticular fibers characteristics can also aid in the diagnosis of certain tumors.

Fibrosis could not be evaluated in 63 biopsies because of a failed reticulin stain, absence of hematopoietic. Most studies have shown that absent or decreased reticulin stain or an abnormal reticulin pattern with widened trabeculae is reliable for the diagnosis of welldifferentiated hcc. Masson trichome a trichrome histology stain is a mixture of three dyes. The reticulin stain in bone marrow biopsies beyond.

View details of cost of test, pretest information and report availability on dr lal pathlabs. Massons trichrome stain figure 2b demonstrates changes in collagen and muscle. Reticulin impregnation in the diagnosis of malignant melanoma. Sep 06, 2017 with the use of stains and dyes, histology allows researchers to visualize particular tissue structures, chemical elements within cells, tissues and even microorganisms.

Demonstrates the reticular fibers in cirrhosis the fibers are disrupted. Elastic fibres provide the elasticity of the blood vessels, lung and skin. This is a helpful stain to use when the diagnosis of hepatocellular carcinoma is being entertained. Reticulin fibers reticulin stain reticulin fibers black demonstrates reticular fibers and basement membrane material mallory ptah martius scarlet blue van gieson reticulin.

Connective tissue stains substance stains component stains possible uses collagen masson trichrome collagen blue green muscle red reticulin blue green fibrin red trichrome stains three colours, for selective demonstration of muscle, collagen fibers, fibrin and erythrocytes. A reticulin stain occasionally helps to highlight the growth pattern of neoplasms. This silver staining process is known as silver impregnation. Reticulin is a type iii collagen found in the basement membrane of many organs and provides structural integrity. Mar 11, 2011 this is a reticulin stain of a liver biopsy. Delicate reticular fibers, which are argyrophilic, can be seen. Reticulin is the special stain used most frequently when facing a diagnosis of possible hcc. Liver anatomy and histology comparative pathology laboratory division of comparative medicine. Historical perspective the story of special stains begins several centuries ago in the early laboratories and workshops of physicians, chemists, botanists and businessmen who were just begin. All hccs showed an abnormal reticulin pattern, which was characterized by a thin layer of reticulin that surrounded each cell nest and was interconnected to form a complete network fig.

The fibers appear black against a gray to light pink background. Pdf files contains the procedure plus a sample container label. Reticulin and cd34 stains for the liver core biopsy of a 55year old patient with history of cirrhosis and well differentiated hepatocellular carcinoma hcc. This histology stain can be helpful for differentiating cellular from extracellular items. Crookham, j, dapson,r, hazardous chemicals in the histopathology laboratory, 2nd ed, 1991, anatech lillie, r. This histology stain is used on connective tissue to visualize collagen and reticular fibers. However, with increasing numbers of slides to be stained, the manual. Conns biological stains, 9th edition williams and wilkins. Reticulin fibers are agyrophilic, meaning that these tissue elements will stain black with a silver solution using the aid of a chemical reducer, which brings the silver into a visible form. It is mainly used in histopathology of the liver, but can also be used to assess abnormalities in the spleen, bone marrow and kidneys.